Genomic landscape and evolution of metastatic chromophobe renal cell carcinoma.

نویسندگان

  • Jozefina Casuscelli
  • Nils Weinhold
  • Gunes Gundem
  • Lu Wang
  • Emily C Zabor
  • Esther Drill
  • Patricia I Wang
  • Gouri J Nanjangud
  • Almedina Redzematovic
  • Amrita M Nargund
  • Brandon J Manley
  • Maria E Arcila
  • Nicholas M Donin
  • John C Cheville
  • R Houston Thompson
  • Allan J Pantuck
  • Paul Russo
  • Emily H Cheng
  • William Lee
  • Satish K Tickoo
  • Irina Ostrovnaya
  • Chad J Creighton
  • Elli Papaemmanuil
  • Venkatraman E Seshan
  • A Ari Hakimi
  • James J Hsieh
چکیده

Chromophobe renal cell carcinoma (chRCC) typically shows ~7 chromosome losses (1, 2, 6, 10, 13, 17, and 21) and ~31 exonic somatic mutations, yet carries ~5%-10% metastatic incidence. Since extensive chromosomal losses can generate proteotoxic stress and compromise cellular proliferation, it is intriguing how chRCC, a tumor with extensive chromosome losses and a low number of somatic mutations, can develop lethal metastases. Genomic features distinguishing metastatic from nonmetastatic chRCC are unknown. An integrated approach, including whole-genome sequencing (WGS), targeted ultradeep cancer gene sequencing, and chromosome analyses (FACETS, OncoScan, and FISH), was performed on 79 chRCC patients including 38 metastatic (M-chRCC) cases. We demonstrate that TP53 mutations (58%), PTEN mutations (24%), and imbalanced chromosome duplication (ICD, duplication of ≥ 3 chromosomes) (25%) were enriched in M-chRCC. Reconstruction of the subclonal composition of paired primary-metastatic chRCC tumors supports the role of TP53, PTEN, and ICD in metastatic evolution. Finally, the presence of these 3 genomic features in primary tumors of both The Cancer Genome Atlas kidney chromophobe (KICH) (n = 64) and M-chRCC (n = 35) cohorts was associated with worse survival. In summary, our study provides genomic insights into the metastatic progression of chRCC and identifies TP53 mutations, PTEN mutations, and ICD as high-risk features.

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عنوان ژورنال:
  • JCI insight

دوره 2 12  شماره 

صفحات  -

تاریخ انتشار 2017